06 - MIC170 4/16/09 Lecture 6 (Thursday, 4/16) How to grow...

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MIC170 – 4/16/09 1 Lecture 6 (Thursday, 4/16) How to grow yeast Growth media Macronutrients; C, N, P(phosphorus) and S(sulfur) Micronutrients; Minerals and Vitamins Yeast extract medium (rich medium) and (synthetic) minimal medium Examples in handout KS-5 Cells grow faster in rich yeast extract medium, which is also easy to prepare. So, why do people use minimal medium? ---Plasmid selection (figure on the right) Strains with amino acid auxotrophy <- transform with a plasmid carrying the wild-type gene as a “selectable marker” Temperature Optimal growth temperature ~30 o C (able to grow at 18~39 o C) Temperature-sensitive mutants --- grow at 25 o C but not at 36 o C They carry a mutant gene expressing a mutant protein sensitive to higher temperature (we will see some examples in later lectures) Oxygen Yeast can obtain energy (ATP) by fermentation without respiration, but O 2 is essential for fatty acid and sterol biosynthesis
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MIC170 – 4/16/09 2 Yeast Molecular Genetics Genetic nomenclature in budding yeast
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06 - MIC170 4/16/09 Lecture 6 (Thursday, 4/16) How to grow...

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