12 - MIC170 Lecture 12(Tuesday 5/12 Genetic dissection of a pathway Correction B Do they function in a linear pathway or independently gene A gene

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MIC170 – 5/12/09 1 Lecture 12 (Tuesday, 5/12) Genetic dissection of a pathway Correction B. Do they function in a linear pathway, or independently? gene A gene A gene B gene B or X X When two genes function in a linear pathway double mutant shows the same phenotype as either of the single mutants of those genes When two genes function independently on the same cellular process double mutant shows a different phenotype from those of the single mutants
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MIC170 – 5/12/09 2 Search for functionally interacting genes Not all genes involved in a biological process may be isolated in the initial genetic screen To discover additional genes, A. Multicopy suppressor screen B. Suppressor mutation screen C. Synthetic lethal screen A. Multicopy suppressor screen Screen a genomic library (in a multicopy vector) that can complement the mutation, looking for genes whose overexpression can complement the mutation You may isolate; Gene with similar function (those that function independently on the same
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This note was uploaded on 06/11/2009 for the course MIC 170 taught by Professor Shiozaki during the Spring '09 term at UC Davis.

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12 - MIC170 Lecture 12(Tuesday 5/12 Genetic dissection of a pathway Correction B Do they function in a linear pathway or independently gene A gene

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