13 - MIC170 Lecture 13(Thursday 5/14 Gene Expression in...

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MIC170 – 5/14/09 1 Lecture 13 (Thursday, 5/14) Gene Expression in Yeast Overview (Fig. 8-2, handout-6) Flow of genetic information in eukaryotes DNA -transcription RNA –translation protein In the nucleus In the cytoplasm (In contrast, transcription and translation are often coupled in bacteria) Structure of eukaryotic mRNAs (Fig. 8.7 from the textbook) prokaryotic mRNAs ------ “polycistronic” No operon in eukaryotes mRNA encodes one polypeptide (protein) ----- “Monocistronic” Also “introns” in eukaryotic transcripts (Fig. 8-51, handout-6) --- introns in primary transcript are removed by “splicing” Poly-A tail is added to the 3’ end of mRNA by poly-A polymerase UTR = UnTranslated Region
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MIC170 – 5/14/09 2 Three forms of eukaryotic RNA polymerase: RNA polymerase I mainly ribosomal RNAs II mRNA III tRNA and other small RNAs These RNA polymerases are subjected to rather different regulations. In this class, we are focusing on transcription by RNA polymerase II.
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This note was uploaded on 06/11/2009 for the course MIC 170 taught by Professor Shiozaki during the Spring '09 term at UC Davis.

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13 - MIC170 Lecture 13(Thursday 5/14 Gene Expression in...

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