16 - MIC170 5/26/09 Lecture 16 (Tuesday, 5/26) Continued on...

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MIC170 – 5/26/09 1 Lecture 16 (Tuesday, 5/26) Continued on “Cell cycle control” Two essential tools for cell cycle study in yeast A. Synchronized culture B. cdc mutants B. cdc mutants c ell d ivision c ycle mutants----arrest at a specific point in the cell cycle Many temperature-sensitive ( ts ) cdc mutants were isolated both in budding yeast (Lee Hartwell) and fission yeast (Paul Nurse). (a) Isolation of cdc mutants i. isolate conditional ( ts ) mutants --- select for mutants that can grow at the permissive temperature (25 o C) but not at the restrictive temperature (37 o C) ii. mutants of interest are those that show a specific block in cell cycle; all cells in the culture arrest at one certain point in the cell cycle with certain cytological landmark e.g., all cells arrest without bud --- cdc mutant that arrests in G1 cdc mutant is deficient in a gene product required to get the cell past the specific point in the cell cycle Fig. 17-25 (handout-8) example: a cdc mutant defective in the initiation of S phase at the restrictive (high) temperature. (b) Characterization of cdc mutants Cloning of the CDC gene by genetic complementation (Fig. 4.32 from Textbook) What kind of protein is encoded? Any known biochemical activity? database search
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MIC170 – 5/26/09 2 Regulatory elements of cell cycle Protein phosphorylation plays important roles in cell cycle regulation
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This note was uploaded on 06/11/2009 for the course MIC 170 taught by Professor Shiozaki during the Spring '09 term at UC Davis.

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16 - MIC170 5/26/09 Lecture 16 (Tuesday, 5/26) Continued on...

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