finalkey - NAME_KEY_ Microbiology 170 Final Exam Spring...

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NAME________KEY ____________ Student ID#_______________________ Microbiology 170 – Final Exam Spring 2008 TOTAL: _____________ /70 Before starting: o The proctor has no knowledge of the exam material – you cannot ask questions during the exam. All necessary information to solve the problems is included in the exam questions. o The lab math question is easy enough to be answered without a calculator. However, the proctor has a calculator you can borrow, if necessary. 1. (1.5 point each) Fill in the blanks to complete the following statements. Mind your handwriting and spelling to receive full credit. A. In eukaryotes, tRNA genes are transcribed by RNA polymerase ( III ). B. In the analysis of gene expression profiles using DNA microarray, ( hierarchical clustering ) is a method to identify groups of genes whose expression is regulated similarly under multiple experimental conditions. C. Lifespan in yeast is measured by counting the number of buds a mother cell can produce, and therefore, called “( replicative ) lifespan”, as opposed to chronological lifespan. D. The yeast mating pheromone receptors belong to the evolutionarily conserved family of ( G protein-coupled ) receptors. All members of this receptor family have seven transmembrane sequences. E. MAPK stands for ( mitogen-activated protein kinase ) and was originally identified as a component of a growth factor signaling pathway in mammalian cells. F(guest lecture question). The end products of glycolysis are ( pyruvate ) and NADH, which are used to produce ethanol in fermentation. 2. (12 points) Choose ONLY ONE most appropriate answer and circle the letter (a-e) beside it for each of the following questions (1) – (6). (1) In eukaryotic gene expression, a. RNA polymerase I is responsible for mRNA transcription b. transcribed mRNA is exported to the cytoplasm for splicing to form mature mRNA c. some exons do not contain any protein-encoding sequence d. both transcription and translation happen in the nucleus. e. mRNAs are usually polycistronic. Q.2 continues to the next page 1. 9 2. 12 3. 4 4. 5 5. 7 6. 5 7. 10 8. 10 9. 6 +2 Grading credit
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2 (2) Heterotrimeric G protein consists of three subunits, G α , G β and G γ . a. G α binds either GDP or GTP. b. G β binds either GDP or GTP. c. G γ binds either GDP or GTP. d. Both G β and G γ bind GDP or GTP. e. All three subunits bind GDP or GTP. (3) MAP kinase is inactivated a. when its conserved Thr residue is dephosphorylated. b. when its conserved Tyr residue is dephosphorylated. c. when either (a) or (b) happens. d. only when both of the Thr and Tyr residues are simultaneously dephosphorylated. e. only when both of the Thr and Tyr residues are simultaneously phosphorylated. (4) The general transcription factors
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This note was uploaded on 06/11/2009 for the course MIC 170 taught by Professor Shiozaki during the Spring '09 term at UC Davis.

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finalkey - NAME_KEY_ Microbiology 170 Final Exam Spring...

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