ks05 - TEL ). The vector is digested with Eco RI and Bam...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Handout KS-5 Examples of yeast growth media
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Cloning large DNA fragments in YACs The vector contains a bacterial origin of replication ( ori ) and a gene conferring ampicillin resistance ( Amp r ), allowing it to be propagated in E. coli . In addition, it contains a yeast origin of replication ( ARS ), a centromere ( CEN ), a selectable marker allowing growth in the absence of uracil ( URA3 ), and two telomeres (
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: TEL ). The vector is digested with Eco RI and Bam HI, yielding two vector arms terminating with telomeres and Eco RI sites. Large fragments of chromosomal DNA prepared by partial digestion with Eco RI are ligated to the vector arms. Yeast cells are then transformed with the recombinant YACs, which replicate as linear chromosome-like molecules....
View Full Document

This note was uploaded on 06/11/2009 for the course MIC 170 taught by Professor Shiozaki during the Spring '09 term at UC Davis.

Page1 / 2

ks05 - TEL ). The vector is digested with Eco RI and Bam...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online