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Unformatted text preview: TEL ). The vector is digested with Eco RI and Bam HI, yielding two vector arms terminating with telomeres and Eco RI sites. Large fragments of chromosomal DNA prepared by partial digestion with Eco RI are ligated to the vector arms. Yeast cells are then transformed with the recombinant YACs, which replicate as linear chromosome-like molecules....
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This note was uploaded on 06/11/2009 for the course MIC 170 taught by Professor Shiozaki during the Spring '09 term at UC Davis.
- Spring '09