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midtermkey - Name_ Microbiology 170 - Midterm Spring 2008...

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Name____________________________ Student ID #____________________ Microbiology 170 - Midterm Spring 2008 TOTAL: /70 1. 18 2. 4 3. 6 4. 8 5. 9 6. 9 7. 10 8. 4 1. (1.5 point each) Fill in the blanks and complete the following statements. Mind your handwriting and spelling to receive full credit. A. In yeast, ( vacuole(s) ) are lysosome-like, membrane-bound organelles that are primarily responsible for hydrolysis of proteins and carbohydrates. B. Like other eukaryotes, yeast cells contain ( mitochondria ), which generate ATP through oxidative phosphorylation. C. Spindle pole bodies are embedded in the nuclear envelope of yeast cells and are equivalent of ( centrosomes ) in animal cells. They function as MTOC. D. During mating of the budding yeast S. cerevisiae , cells show polarized elongation towards the mating partner. This specialized cell elongation is called ( shmoo(ing) ). Cytoplasmic fusion of two mating cells is followed by nuclear fusion, forming a diploid cell called a ( zygote ). E. When starved of nutrients, haploid yeast cells enter the ( stationary ) phase, where they have reinforced cell wall and accumulate glycogen. Those cells are resistant to harsh environments. F. In S. cerevisiae , vegetatively growing haploid cells show the “( axial )” budding pattern, where new buds start near the previous division site on mother and daughter cells. This is in clear contrast to “( bipolar )” budding seen in a / α diploid. G. When available nutrients are limited, a / α diploid initiates meiosis and form an ( ascus ), which contains four haploid spores. H. Most of S. cerevisiae strains found in nature are homothallic and can switch their mating type. On the other hand, many of lab strains are heterothallic and do not switch their mating type, due to a mutation in the ( HO ) endonuclease gene. I. ( (Synthetic) minimal ) medium is usually used to selectively grow yeast cells carrying a plasmid. J. Plasmid vectors used in the yeast study can replicate in both yeast and E. coli , and they are often called “yeast– E. coli ( shuttle ) vectors”. +2 Grading credit
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2 2. Lab math Your lab supervisor asked you to prepare 100 ml of solution Z, following the lab recipe. The lab recipe book says you should dissolve 120 g of NaH 2 PO 4 (anhydrous) in water to make 1 liter of solution Z. However, the lab seems to have only NaH 2 PO 4 •H 2 O (monohydrate; FW = 138.0) in the chemical cabinet and you cannot find anhydrous NaH 2 PO 4 powder anywhere. Just in case you do not remember, FW of H2O is 18.0. (1) (
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midtermkey - Name_ Microbiology 170 - Midterm Spring 2008...

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