tributyrin media.docx - Principle: Tributyrin agar is a...

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Principle:Tributyrin agar is a differential medium that tests the ability of an organism to produce anexoenzyme, called lipase, that hydrolyzes tributyrin oil. Lipases break down lipids (fats).Tributyrin oil is a type of lipid called a triglyceride. Other lipase tests use different fat sourcessuch as corn oil, olive oil, peanut oil, egg yolk, and soybean oil.Some bacteria have the ability tohydrolyse lipids (fats) to glycerol and fatty acids, as they possess the lipolytic enzyme ‘lipase’.These bacteria are called lipolytic bacteria. While the lipids form an emulsion, when dispensed inagar, producing opacity, their hydrolysed end products, glycerol and fatty acids, do not form suchemulsion with agar, for which they do not produce such opacity; rather produce transparency.In the lipid hydrolysis test, the test bacteria are grown on agar plates containing tributyrin as thelipid substrate. Tributyrin forms an emulsion, when dispensed in the agar, producing an opaquemedium.If the bacteria has the ability to hydrolyse lipids, its colonies hydrolyse the tributyrin in the

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Lipid

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