Chapter 6 - Chapter 6 Gene Interaction I Genes act by...

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Chapter 6: Gene Interaction I) Genes act by controlling cellular chemistry A) Mutations can causes defects in metabolism (general set of chem.. reactions in organism) B) One-gene-one-polypeptide hypothesis: a gene encodes the physical structure of a protein, which dictates its function--Affect function of enzymes C) Gene interaction in synthetic pathways (chain of enzymatic conversions that creates essential nutrients) II) Inferring gene interactions A) Treat cells with mutation causing agents—confirm single-gene inheritance B) Test mutants to determine total number of gene loci taking part and which mutations are alleles on the same gene. Done through Complementation test— determine if two mutations belong to same gene 1) Can only be applied to recessive mutations 2) Cross two homozygous mutant lines to produce heterozygote a) If wild-type—mutations complement one another and are on different genes (Complementation—production of wild-type phenotype when two haploid genomes bearing diff. recessive mutations are united in same cell)
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