Endospore Micro 312- 2:18:19.docx - Endospore Discussion Kira Kopec Micro 312 Room 301 Monday In this lab the purpose was to isolate endospore-forming

Endospore Micro 312- 2:18:19.docx - Endospore Discussion...

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Endospore Discussion Kira Kopec Micro 312, Room 301 Monday February 18, 2019 In this lab, the purpose was to isolate endospore-forming bacteria from soil through the process of pasteurization. After pasteurizing the sample, it is expected that you should be able to observe a sample of Gram-positive endospore-forming bacteria growing on the nutrient agar (NA) as all vegetative cells will have been killed after heating. To observe, a gram stain and wet mount will be taken from the post-pasteurization plate to record endospore growth. These results are expected after pasteurizing bacteria in nutrient broth as only Gram-positive bacteria containing endospores will be able to survive the pasteurization process. Gram-positive bacteria can produce endospores under environmental stressors, which includes being able to withstand excessive heat, cold, drying, and starvation; Endospores produce in times of nutrient deprivation (low nitrogen and carbon) After mixing soil in nutrient broth (NB), a loopful of the mixture was streaked onto one
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