Biofilms Discussion.docx - Biofilms Kira Kopec Micro 312 Room 301 Microscope Slide Culture The purpose of this lab was to observe the communication of

Biofilms Discussion.docx - Biofilms Kira Kopec Micro 312...

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Biofilms Kira Kopec Micro 312, Room 301 March 22, 2019 Microscope Slide Culture The purpose of this lab was to observe the communication of bacteria to produce biofilms in slide cultures containing TSB + 0.2% glucose. In one petri dish, an environmental sample (lettuce) was placed onto the slide following the addition of 10 mL of TSB + 0.2% Glucose. The purpose of glucose is to allow for extra sugars to be available to the bacteria so that they can flourish and form an extracellular polymer substance (EPS). In the second petri dish, 0.50mL of Pseudomonas aeruginosa is added onto the slide containing 9.50mL of the TSB- glucose mixture. After given the chance for the bacterium to grow, flow-through gram stains are performed on the two slides and viewed at TM=400x and 1000x. The purpose of a flow-through gram stain is because dryness destroys the biofilm as it is a complex arrangement. Due to its complexity, the integration of a special gram stain allows the organism to be viewed while still wet. It is expected that biofilms will form on the slides as P.aeruginosa
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