Micro Lab #4.docx - ELN Example(Nicole Coan...

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ELN Example (Nicole Coan) Day #4: (Traditional Diagnostics) (September 24, 2018) Vocabulary: Anaerobic -a metabolic process that does not require oxygen Confirmatory test -second analytical procedure used to identify the presence of a specific drug or metabolite. Independent of the initial screening test and uses a different technique and chemical principle from that of the initial test to ensure reliability and accuracy. Durham fermentation tube -small inverted tube placed inside the phenol red broth vial, used to assess gas production Heterofermentative -producing a mixture of products, typically lactate, ethanol, and CO2 from the fermentation of glucose Homofermentative -simple fermentation in which lactic acid is only product Lab 1: Gram Stain Introduction To perform a gram stain on your own unknown to check that it is a pure culture. Protocol 1. Light the Bunsen burner with a striker and take the inoculating loop and put it under the flame until it glow red. This sterilizes the loop. 2. Make sure to cool the flame so you do not kill the bacteria. To do this put it in water. Then using the loop transfer some water to a microscope slide. 3. Use the bacteria, in this case your unknown, that has been grow on a agar plate and transfer it to a microscope slide on top of the water to mix it thoroughly. Let it air dry. 4. Take a cloth pin to hold the microscope slide with the water and single isolated colony on it. 5. To perform heat fixation, move the dried slide three times over the Bunsen burner flame. 6. Take a staining rack and gentle place the slide on the rack.
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ELN Example (Nicole Coan) 7. Flood the slide with crystal violet (primary stain) and allow it to dry for 1 minute. Get excess off by tilting the slide letting it run off.
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  • Fall '19
  • Growth medium, Agar plate, Petri dish, Nicole Coan

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