ANFS310 Chromosome Structure DA.docx - \Chromosome Structure Electrophoresis Data Analysis Please TYPE ALL ANSWERS – double spaced Your name should be

ANFS310 Chromosome Structure DA.docx - Chromosome...

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\Chromosome Structure & Electrophoresis Data Analysis Please TYPE ALL ANSWERS – double spaced. Your name should be on every page *using a header is an easy way to do this* You may choose to print this assignment, and turn it in according to your lab date OR upload it onto Canvas before coming to your lab. All questions, except #7, must consist complete sentences. Figure 1. DNA from wheat germ was digested with micrococcal nuclease and the resulting fragments were separated by agarose electrophoresis on a 1.5% gel. Lanes 2, 3, 4 and 5 contain samples of DNA that were digested with 20 uL of nuclease. The length of the incubation time of the enzyme with the samples varied. Lane 1 contains a 100 bp ladder.
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Nucleosome Structure Data Analysis 1. Referring to Figure 1: Thoroughly explain the dark smear in the upper portion of lane three. The dark smear in the upper portion of lane three indicates that this lane contains the largest amount of undigested enzyme in the well. The smear also shows that this lane contains many long DNA fragments and histones of various lengths that cannot be resolved. 2. Referring to Figure 1: Thoroughly explain the dark band in the well of lane three. The dark band in the well of lane three indicates sample DNA and histones that were not digested by the nuclease. The dark band shows that the 2 of 6 3 Nucleosomes + Linker ~530 bp 1 Nucleosome + Linker ~165 bp
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Nucleosome Structure Data Analysis sample DNA and histones are too large to enter the gel. The darkness of the well may also indicate that it was overloaded, meaning sample DNA was put into the well and all of it cannot be pulled through the gel by the current.
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