CABUGATAN BACTE.docx - Cabugatan Bejan S BSMT 3 Bordetella...

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Cabugatan, Bejan S. BSMT 3 July 03, 2019 Bordetella pertussis and Legionella Bordet-Gengou agar Potato-glycerol–based medium enriched with 15%-20% defibrinated blood; contaminants inhibited by methicillin (final concentration of 2.5 µm/mL) Isolation of Bordetella pertussis and Bordetella parapertussis Regan Lowe Charcoal agar supplemented with horse blood, cephalexin, and amphotericin B Enrichment and selective medium for isolation of Bordetella pertussis Buffered charcoal-yeast extract agar Yeast extract, agar, charcoal, and salts supplemented with L- cysteine HCl, ferric pyrophosphate, ACES buffer, and α-ketoglutarate Enrichment for Legionella spp. Supports the growth of Francisella and Nocardia spp. Buffered charcoal-yeast extract (BCYE) agar with antibiotics BCYE supplemented with polymyxin B, vancomycin, and ansamycin, to inhibit gram- negative bacteria, gram-positive bacteria, and yeast, respectively Enrichment and selection for Legionella spp CAMPYLOBACTER Campy-blood agar Contains vancomycin (10 mg/L), trimethoprim (5 mg/L), polymyxin B (2500 U/L), amphotericin B (2 mg/L), and cephalothin (15 mg/L) in a Brucella agar base with sheep blood Selective for Campylobacter spp Schaedler agar Peptone and soy protein base agar with yeast extract, dextrose, and buffers; addition of hemin, L-cystine, and 5% blood enriches for anaerobes Nonselective medium for the recovery of anaerobes and aerobes Selective for Campylobacter and Helicobacter spp. Skirrow agar Peptone and soy protein base agar with lysed horse blood; vancomycin inhibits gram- positive organisms; polymyxin B and trimethoprim inhibit most gramnegative organisms Selective for Campylobacter spp. Campylobacter thioglycollate broth Contains vancomycin (10 mg/L), trimethoprim (5 mg/L), polymyxin B (2500 U/L), amphotericin B (2 mg/L), and cephalothin (15 mg/L) in a Brucella agar base with sheep blood Selective for Campylobacter spp . Cefoperazone, vancomycin, Blood-supplemented enrichment Selective medium for isolation of
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amphotericin (CVA) medium medium containing cefoperazone, vancomycin, and amphotericin to inhibit growth of most gram-negative bacteria, gram-positive bacteria, and yeast, respectively Campylobacter spp.
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