ANFS310 DNA Isolation Study Questions.doc - DNA Isolation Study Questions 1 What is the function of sodium dodecyl sulfate(SDS in isolation of bacterial

ANFS310 DNA Isolation Study Questions.doc - DNA Isolation...

This preview shows page 1 out of 1 page.

DNA Isolation Study Questions 1. What is the function of sodium dodecyl sulfate (SDS) in isolation of bacterial DNA? The function of sodium dodecyl sulfate (SDS) in isolation of bacterial DNA is to lyse the cells. 2. What is the function of trypsin (or protease) in isolation of bacterial DNA? The function of trypsin in the isolation of bacterial DNA is to digest cellular proteins at 60°C and inactivate other enzymes. 3. What is the function of the EDTA-buffer? The two main functions of the EDTA-buffer are the inhibition of DNase and maintaining the pH. 4. It is necessary to raise the temperature to 60 C when purifying DNA to inactivate some enzymes. What enzymes need to be inactivated? Why is this step important? The enzymes that need to be inactivated are cellular proteins (DNAse). This step is important because proteins are the most common contaminate of DNA, so it is important that all the
Image of page 1

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Ask Expert Tutors You can ask 0 bonus questions You can ask 0 questions (0 expire soon) You can ask 0 questions (will expire )
Answers in as fast as 15 minutes