micro practical 1.docx - LAB EXAM#1 REVIEW SHEET v.2 – Fall 2018 BIOL 2240 – LABs This is a study guide NOT an absolute list of everything that was

micro practical 1.docx - LAB EXAM#1 REVIEW SHEET v.2 –...

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LAB EXAM #1 REVIEW SHEET v.2 – Fall 2018 BIOL 2240 – LABs This is a study guide, NOT an absolute list of everything that was covered. For the exam there will be both a written component and practical stations where you get to demonstrate your knowledge. Find below a nearly comprehensive list of terms and concepts that you should be familiar with for the first lab test . Test coverage includes: Basic Microbiology Laboratory Safety (pgs. 1-6), Lab Exercises and handouts. All scientific names should be spelled properly and formatted correctly (underlined and with Genus name capitalized) E. coli MICROSCOPE AND MICROSCOPIC MEASUREMENT Recognize and name all microscope parts and their uses (4 objective lenses, ocular/eyepieces…) Proper procedure when focusing the microscope and viewing a slide. (be able to write a step by step description and be able to demonstrate under timed exam conditions). 1. Turn microscope on 2. Place slide with specimen pointing up toward the oculars 3. Adjust as needed the y and x axis knobs to bring specimen into the right position 4. Use the coarse adjustment to bring specimen into focus first 5. Once you can roughly see the specimen, use the fine focus knob to zoom the rest of the way in 6. Always start on 4x and work your way up (4x,10x,40x,100x) 7. Once you go to a higher power, only use the fine focus knob Proper use of the fine adjustment knob, for example, the order of the colored crossed threads viewed at 10X and 40 X . the fine adjustment knob is used to get a sharp focus, and used only on higher powered lenses.// cross thread order is yellow, blue, red (lightest color on top) Know pitfalls of using the High-dry lens (40X). E.g. Do you use the coarse focus knob when the 40X is in position? The high dry lens has a narrower field of view than the lower power lenses. The coarse focus knob should not be used when the 40x is in position, only the fine knob can be adjusted Immersion oil - what is its function? Reduces light refraction (loss of light) and improves resolution. (enhances resolving power of the microscope) Do you use the coarse focus knob when the 100X is in position? NO, because there is such a small working distance between the 100x and slide. If used you risk ruining the slide or the lens Proper use of oil immersion and how to avoid problems associated with it use. E.g. How to avoid getting oil on the 40X? - Place a drop of immersion oil directly on top of the light spot - Avoid getting oil on the 40x by always working your way up. Always start at 4x,10x,40x,100x and never go backwards. If you have to go backwards, you must clean the slide and lens Determine total magnification (objectives lens power X ocular lens power). Total magnification = objective lens power x ocular lens power, which is usually 10x Know at least two ways to adjust light intensity on the microscope and the order you should adjust them.
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