Introduction to the spectrophotometer.pdf - Measuring Concentrations How do you determine the concentration of a solution a You have prepared b You have

Introduction to the spectrophotometer.pdf - Measuring...

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Measuring Concentrations How do you determine the concentration of a solution: a) You have prepared b) You have not prepared (e.g., a drinking water sample)
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Analytical instruments Light transmittance Absorbance Conductivity
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Spectrophotometer A beam of light filtered to a specific wavelength, or a narrow range of wavelengths through a solution sample onto a light detector Concentration of the solution can be determined based on the wavelengths and the amount of light absorbed/transmitted. Source: . Accessed Sep 13, 2016.
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Spectrophotometer (cont’d) Cuvette sample holder Light detector measures: Transmittance - the amount of light passed through the solution sample A bsorbance - the amount of light absorbed by the solution sample
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Reference blank There may be other substances in the solution sample that absorb the same wavelengths as the substance being analyzed. compare the absorbance/transmittance of the solution sample to a reference blank Source: . Accessed Sep 13, 2016.
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