MIC205_Chp-4_Teamwork-Student ANSWERS.docx - Chapter 4 Teamwork Team Number Questions 1-9 Fill in the concept map below with the following words A

MIC205_Chp-4_Teamwork-Student ANSWERS.docx - Chapter 4...

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Chapter 4 Teamwork Team Number: _________________________ Questions 1-9: Fill in the concept map below with the following words A. Decolorizer B. Gram-positive bacteria C. Purple D. Iodine E. Ethanol and Acetone F. Safranin G. Gram-negative bacteria H. Primary stain I. Thin peptidoglycan layer J. Inner membrane 1. _________________ 6. ____________________ 2. _________________ 7. ____________________ 3. _________________ 8. ____________________ 4. _________________ 9. ____________________ 5. _________________ Questions 10-15: For each description about acid fast staining, match the appropriate reagent (each step can be used more than once): A. Methylene blue B. Carbolfuchsin C. Hydrochloric acid 10. Stains all cells __________ 11. Stains acid-fast cells ________ 12. Primary stain __________ 13. Counterstain __________ 14. Decolorizer ___________ 15. This stain is removed during decolorizing __________
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Questions 16-24: What are we observing? Indicate “True” for all that apply, and “False” for those that don’t. Questions 26-32: Your microbiology lab instructor has given you a mixture of gram positive and gram negative cells to practice your gram staining technique. After you complete the stain, you observe only pink colored cells under the microscope. Which steps may have gone wrong? Indicate “True” for
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  • Spring '08
  • wash
  • Bacteria, gram-negative bacteria, A. Decolorizer, F. Safranin

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