Topic7.pdf - Topic 7 DNA Replication 1 Describe the...

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Topic 7: DNA Replication 1. Describe the mechanisms of DNA replication, including the steps and molecules involved. Explain why this is a semiconservative process and how this process results in the synthesis of a nearly perfect copy of the entire genome. 2. Compare replication in prokaryotes and eukaryotes, particularly focusing on the unique issues that arise in the large, linear chromosomes of the average eukaryotes genome. 3. Survey how biologists have harnessed replication as an experimental tool in molecular genetics. Include an explanation of the following methods: Polymerase Chain Reaction, Gel Electrophoresis, Sanger Sequencing, and Illumina Fluorescent Sequencing.
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Topic 7: Big Question How are precise copies of a genome made?
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Our first topic on mechanisms…. Think about these in a narrative arc, rather than trying to memorize a list of disjointed facts! 3
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DNA replication Why does DNA replicate? When does DNA replication take place? What are the goals of DNA replication? What is the mechanism? Stages Molecules necessary Prokaryotes vs. eukaryotes 4
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DNA replication is a semiconservative process: 5
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Building blocks of replication DNA template dNTPs (dATP, dGTP, dCTP, dTTP) Enzymes DNA polymerase Primase Enzymes for unwinding DNA 3’-OH end to build upon Energy (ATP) 5’ 5’ 3’ DNA replication explained in a fun way: 6
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Initiation – where does replication start? Origin of replication During initiation, start opening the “bubble” 7
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