Frog Heart Lab report - Title Frog Heart Name Esther Georges Partners Names Class/Section BIO 205 Instructor Faiza Peetz Date of Experiment March 19

Frog Heart Lab report - Title Frog Heart Name Esther...

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Title: Frog Heart Name: Esther Georges Partners Names: Class/Section: BIO 205 Instructor: Faiza Peetz Date of Experiment: March 19. 2019 Esther Georges
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Prof. Faiza Peetz March 19, 2019 Frog Heart ABSTRACT The purpose of this lab was to examine the effects of temperature and various drugs on specifically contraction strength and heart rate. The contraction and electrogram signals were measured with a force transducer attached that was attached to the thread to the apex of the heart. There are many external factors that can affect the cardiac behavior and output. This then leads to make changes in the heart rate, the size and strength of the contraction, and even down to the way that action potential is produced. This lab shows how neurotransmitters or temperature can act to alter the performance of the entire heart. It is important to understand that neurotransmitters are chemicals that activate nerve cells and allow them to communicate with other nerve and muscle cells. It is able to activate the autonomic nervous system to either cause the heart rate to speed up or slow down. The frog was pithed and the heart was exposed, and along with the naturally occurring neurotransmitters in the body that were used, the other drugs we used to measure the reaction of the heart when it is influenced externally. The force transducer was the method in which we measure the action while using the electrodes to record the pulse rate and the amplitude of the heart in between the times that the drugs are administered. To be brief, the experiment will observed the effect of the drugs on the chemical and mechanical interaction of the contraction strength of the cardiac muscles. The ECG will allow us to record it properly and accurately pinpoint the anticipated effect of the drugs. MATERIALS Biopac Student Lab System Force Transducer (SS12LA includes S-hooks) Ring Stand Tension adjuster (HDW100A or equivalent), Small hook for connecting thread to the heart Thread (non-stretch nylon or equivalent) , Dissection Pan, Dissection Kit Live frog BSL PRO template file: “a04.gtl” Drug preparations: (all drugs to be made in Ringer's solution as a solvent); calcium chloride (10 mM); verapamil (10 μM), digitalis (10μM); acetylcholine (5μM), atropine
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(50 μM); nicotine (10 mM); potassium chloride (30 mM); epinephrine (adrenalin chloride) (5 μM); METHODS 1.
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  • Fall '15
  • Dr. Charles Kramer

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