Topic13.pdf - RNA Based Regulation 1 Characterize the ways...

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RNA Based Regulation 1. Characterize the ways in which RNA molecules can regulate gene expression by affecting transcription or translation. 2. Distinguish between mechanisms of RNAi. 3. Investigate how RNA-mediated regulation can be co-opted in biotechnology.
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Big Question: Are proteins the only molecules that can act in trans- to affect expression of genes?
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1.Changes in transcription 2.Changes in physical or chemical structure of chromatin 3.RNA-based mechanisms of regulation in both prokaryotes and eukaryotes acts during or after transcription uses RNAs that are never translated 3 Regulation Overview: WARNING: no one book chapter, so I’ll include page numbers as we go
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Mechanisms of RNA-mediated regulation Alternative splicing RNA degradation mRNA secondary structures Antisense RNA (sRNA) RNAi miRNA siRNA
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We’ve touched on RNA based regulation before: Alternative splicing Via binding of SR proteins that bind specific sequences in the pre-mRNA and influence the choice of splice sites. Affects the functionality of the protein product Can have huge phenotyptic effects, i.e., Tra in Drosophila melanogaster RNA stability Ribonucleases break down cellular mRNA Timing may vary depending on features of mRNA Sequence motifs in UTR or coding 5
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RNAmovies program mRNA Secondary Structures Alternate secondary structure can arise Most are straightened out by helicase ahead of the ribosome Some secondary structures may either decrease or increase transcription or translation Attenuation Riboswitches 6
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