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Unformatted text preview: 3 = digestion with restriction enzyme 4 = ethidium bromide 5 = radioactive probe a. 3, 2, 4, 5, 1 b. 3, 4, 2, 5, 1 c. 3, 2, 5, 4, 1 d. 2, 4, 3, 5, 1 5. The DNA probe will bind to identical nucleotide sequences on the test sample. a. True b. False 6. Which of the following is a difference between genomic DNA and cDNA? a. cDNA has only introns b. cDNA is made by reverse transcriptase c. cDNA libraries require chromosomal mRNA isolation d. cDNA contains regulatory regions Name: 7. A radiolabeled nucleotide can be added to the 3 end of a probe using which of the following? a. Polynucleotide kinase b. Fusion screening c. Terminal transferase d. Conjugation 8. How can a cell protect its genome from digestion from its own restriction endonucleases? a. Methylation b. Radiolabeling c. Phosphorylation d. Use of antibodies 9. DNA polymerase preferentially adds dNTPs over ddNTPs in sequencing. a. True b. False...
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