Quiz_5_key - 3 = digestion with restriction enzyme 4 =...

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Name: Quiz #5 1- Antibiotic resistant genes in plasmid are important for: a. amplification of DNA b. insertion of DNA c. selected growth of a particular plasmid carrying this gene d. efficiently expression of the target cDNA e. none of the above 2- The following substrates are best for restriction endonucleases (I told you I am going to ask you this question) a. single stranded DNA b. double stranded DNA c. mRNA d. double stranded RNA e. DNA/RNA hybrids f. none of the above g. only a, b and d 3- The purpose of the Southern Blot test is to: a. look for a specific nucleotide sequence in the DNA being tested b. to determine how closely two organisms are related c. to identify the size of the fragment that contains the sequence d. a and c e. a, b, and c 4- The steps involved in the Southern Blot test should be performed in the following order 1 = x-ray film 2 = electrophoresis
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Unformatted text preview: 3 = digestion with restriction enzyme 4 = ethidium bromide 5 = radioactive probe a. 3, 2, 4, 5, 1 b. 3, 4, 2, 5, 1 c. 3, 2, 5, 4, 1 d. 2, 4, 3, 5, 1 5. The DNA probe will bind to identical nucleotide sequences on the test sample. a. True b. False 6. Which of the following is a difference between genomic DNA and cDNA? a. cDNA has only introns b. cDNA is made by reverse transcriptase c. cDNA libraries require chromosomal mRNA isolation d. cDNA contains regulatory regions Name: 7. A radiolabeled nucleotide can be added to the 3 ’ end of a probe using which of the following? a. Polynucleotide kinase b. Fusion screening c. Terminal transferase d. Conjugation 8. How can a cell protect its genome from digestion from its own restriction endonucleases? a. Methylation b. Radiolabeling c. Phosphorylation d. Use of antibodies 9. DNA polymerase preferentially adds dNTPs over ddNTPs in sequencing. a. True b. False...
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This homework help was uploaded on 04/01/2008 for the course BIOL 2104 taught by Professor Dcimini during the Spring '08 term at Virginia Tech.

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Quiz_5_key - 3 = digestion with restriction enzyme 4 =...

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