LabTechsExperiment7.docx - 1 Zainab Makki NFS 5140-005,006 Lowry Protein Assay Experiment 7 Abstract Proteins function for almost all metabolic

LabTechsExperiment7.docx - 1 Zainab Makki NFS 5140-005,006...

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1 Zainab Makki NFS 5140-005,006 October 28, 2013 Lowry Protein Assay Experiment 7 Abstract: Proteins function for almost all metabolic reactions in the human body. They are formed from various combinations of the 20 amino acids, and folded to primary, secondary, tertiary, and quaternary structures. There are numerous lab techniques in order to determine the protein type. Proteins can be determined by many methods such as Kjeldahl Method, Dye binding Method, Biuret Method, Lowry Method, Ultraviolet Method, Fluorescence Method, Bio Rad assay. In this lab we used the Lowry Protein Assay for determining proteins. We had to remove most results that were outliers, including all of the 750 concentration wells, as well as a few others. This may be why the graph is a little off around those values. For some reason, our group had a great deal of experimental error, therefore eliminating most of our usable data. Overall we had an increase in absorbance, with concentration. Introduction: Proteins are made from single chains of amino acids. There are 20 common amino acids that are found in natural protein. Proteins are the working molecules of the cell; without proteins the cell would not survive. They carry main functions of the cells such as catalyzing a vast range of chemical reactions, providing structure to the cell, controlling the flow of material through the cell membrane, regulate concentrations of metabolites, act as sensors and switches, cause motions, and control gene function. They carry out all the activities encoded with genes. They build the structure of the cell and carry important cellular functions such as processing materials by catalyzing intracellular or extracellular chemical reactions.
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2 Zainab Makki NFS 5140-005,006 October 28, 2013 There are three different types of proteins, globular, fibrous, and conjugated proteins. Globular proteins are in tertiary structure and spherical. They are soluble in water. Enzymes, oxygen transporting proteins (hemoglobin), hormones, albumins and globulins are all globular proteins. Fibrous proteins are molecules such as collagen and elastin, in meat and poultry.
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  • Fall '14
  • DonaldBarnes
  • Zainab Makki

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