Transduction Transduction is a process of gene transfer by bacteriophage vectors. The altered bacteriophage can act as a vector for gene transfer to new host. Recombination Following gene transfer, there are several possible outcomes for DNA. If the transferred gene is on a replicon, F’ for example, it is replicated independent of its host chromosome. But if the transferred gene lacks a replicon, it is either destroyed or integrated into the host chromosome by homologous recombination. To be integrated as an episome, it requires complete or near identity to the host gene. Homologous recombination is common is prokaryotes but very rare in eukaryotes. Smith and Arber are credited with discovered the Restriction of Modification system of DNA. Smith and Arber discovered the restriction of endonucleases, which cleave DNA at specific sequences. Every bacterium consists of many strains. The DNA of a phage previously grown in E. coli C was modified to resemble C’s DNA. When the C modified phage DNA is
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This note was uploaded on 04/01/2008 for the course MICRB 201 taught by Professor Sodeinde,olanrewa during the Fall '08 term at Penn State.