Translation2 - ~10kVolts in the presence of DNA. The shock...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Translation Translation is the uptake of naked DNA. Some bacteria, mostly those gram-positive, are naturally competent in the uptake of naked DNA. Griffith’s experiments on the transforming principle are an example. However, many bacteria must be induced into the uptake of DNA through the application of chemicals, heat shock, or electroporation. Electroporation is the exposure of cells to
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ~10kVolts in the presence of DNA. The shock punches holes in the membranes and allows DNA to enter. Transformation is standard procedure for the cloning of genes. In order to be successful, the DNA must share similarities with the bacterial chromosome or the DNA will be degraded....
View Full Document

Ask a homework question - tutors are online