BMB 212 Practice Problems - BMB 212 Practice Problems Gas...

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BMB 212 Practice Problems Gas Chromatography Gas chromatography is comprised of an inert gaseous mobile phase and a non-volatile liquid stationary phase coated onto an inert solid support. The carrier gas is the pressurized gaseous mobile phase which continuously sweeps through the packed column. The packed column is held at an elevated temperature. Helium was our carrier gas. The flow rate of the carrier gas plays a role in the separation of the components; a slower flow rate allows more vapors to interact with the stationary phase and can yield better resolution. However, if the flow rate is too slow then diffusion may occur. A faster flow rate offers quick data analysis but less opportunity for the vapors to interact with the stationary phase, which can disallow for the separation of components. The injection port is heated to 220 degrees Celsius in order to vaporize the injected sample and introduce it into the carrier gas for transport through a heated column.
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