Micro 2020 Lab Mid Term Exam Study Guide_Fall 2018.docx - Micro 2020 Lab Mid Term Exam Study Guide Note that everything that has been covered in lab as

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Micro 2020 Lab Mid Term Exam Study Guide Note that everything that has been covered in lab as well as in your lab manual is fair game up to endospore stain (lab session 1 through 6) 1. Common Aseptic Transfer and Inoculation Method i. Know how to properly discard material (look at your lab rules) Test tubes: discarding racks Cotton swabs slides, razors : sharps container Biohazard bin : solid media, napkin contaminated “clean gloves”: ii. Sterility : removing all microbes iii. Know how to sterilize the different types of equipment used i.e. i. Tubes : flames mouth ii. Bottles : open them and close quick iii. Agar plates : open the lid at an angle to minimize iv. Loops and needles : flame until turns red, entire wire (allow to cool down) iv. Subculture : transfer of bacteria from one media to another v. Know the different types of media that were used i. Agar slant : solid at room temperature (slant: ideal if you want to store) ii. Agar Plate : used if trying to isolate or if you look at morphology iii. TSB broth : liquid at room temperature (ideal for large numbers in bacteria population or if you want fresh cultures. 2. Streak Plate Method of isolation i. pure culture : contain only one organism (bacteria from the setup table) ii. mixed colony : more than one organism (sample from nature: water, soil…) iii. Colony : mass of bacterial cells seen without a microscope iv. Quadrant streak : used if high cell density (high concentration will be in 1 st quadrant. The lowest concentration will be the 4 th quadrant) v. Zig Zag : used for low cell density 3. Ubiquity of Microorganisms i. organism to be ubiquitous : found in wide range of environment ii. pathogens: can cause infections iii. opportunistic pathogens: wait for a decrease in the efficiency of immune system iv. nonpathogenic bacteria : can’t cause infections (breakdown of nutrients) v. reservoir : area where microbes reside and can cause infection.
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