Short Report .docx - Patel 1 Direct and Indirection Determination of Analytes Using Fluorescence Spectroscopy Chandni Patel Patel 2 Purpose[2 The

Short Report .docx - Patel 1 Direct and Indirection...

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Direct and Indirection Determination of Analytes Using Fluorescence Spectroscopy October 30, 2018 Chandni Patel Patel 1
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Purpose [2]: The purpose of this lab was to find the concentration using the emission intensity of the unknown. Data Tables: Data Table 1: Direction Determination Calibrant Concentration (ppm) Fluorescence Emission 0.5 196.8 1.0 385.6 1.5 555.2 2.0 700.8 2.5 894.4 Diluted Unknown: 0.125 228.8 Figure 1: Direct Determination Graph 0.000 0.500 1.000 1.500 2.000 2.500 3.000 0 100 200 300 400 500 600 700 800 900 1000 f(x) = 299.18 x + 113.4 R² = 0.97 Direct Determination Calibrant Concentration Fluorescence Emission Equations: Calculating quinine: C 1 V 1 = C 2 V 2 (1) Calculating Concentration: Patel 2
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y = mx + b (2) Results & Discussion: This lab was about fluorescence spectra. Fluorescence spectra can be used when looking for the emission and excitation wavelengths individually. It is also used for finding multiple wavelengths of both emission and excitation. Fluorescence and phosphorescence are two of the main techniques that uses luminescence. This is because they both are high in sensitivity.
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