HersheyChase - The Hershey-Chase Blender Experiment a...

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The Hershey-Chase Blender Experiment • a simple and classic experiment! • performed by Alfred Hershey and Martha Chase at Cold Spring Harbor Laboratory in New York in  1952 • published: AD Hershey and M Chase “Independent functions of viral protein and nucleic acid in  growth of bacteriophage” Journal of General Physiology 36: 39-56 (May 1952) • using Escherichia coli (E. coli) bacteria and T-group bacteriophages (phages or bacterial viruses) • cultures of E. coli were grown in media that contained as the only source of sulfur, radioactive S-35  sulfur in sulfate form • and cultures of E. coli were grown in media that contained as the only source of phosphorus,  radioactive P-32 phosphorous in phosphate form • such bacterial cells will have all their sulfur and phosphate, respectively, radioactive • infect these radioactive bacteria with phages
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