Bio104-SordariaLabAbstract

Bio104-SordariaLabAbstract - Michael Glamore Sordaria...

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Michael Glamore Sordaria Abstract The investigation of the organism with scientific name Sordaria flimcola has proved to be a strong example of the power of basic genetic principles. In the first part of the experiment, the black wild type strain was crossed with two mutant strains, tan and grey. This is aimed to determine the gene locations relative to their respective chromosome. Additionally, statistical analysis of a grey vs. tan cross should provide information to the likelihood of these genes being linked. The entire experiment relies on the peculiar way that this multicellular fungus produces haploid ascospores. As a diploid cell undergoes meiosis into four daughter cells and experiences mitosis it keeps the eight ascospores in order inside an ascus. One can squash the Perithecia to reveal these asci. By analyzing the order of the ascospores’ phenotype in the ascus by tetrad analysis, it can be determined if crossing over has occurred.
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