100%(1)1 out of 1 people found this document helpful
This preview shows page 1 - 2 out of 3 pages.
Bronx High School of ScienceScience DepartmentMs. Boone/Ms. Stern, Instructors Nutritional Science - Vitamin C LabObjective:To test various types of juices for ascorbic acid content.Introduction:Ascorbic acid (vitamin C) is a water-soluble vitamin found in relatively high concentrations in citrus fruits such as oranges, grapefruits and lemons, as well as vegetables of the cabbage family. In its active form, it is in a reduced state, but it can be readily oxidized (lose hydrogen) to become dehydroascorbic acid. In the oxidized form it does not act as a vitamin.Oxidation2HC6H8O6(Ascorbic Acid) C6H6O6(Dehydroascorbic Acid)2HReductionThis property provides a method for the quantitative analysis of ascorbic acid. Using the schematic below, you can see that the amount of reduction of blue indophenol to colorless is proportional to thenumber of hydrogen’s contributed through the oxidation of ascorbic acid. Therefore, by counting thenumber of drops of ascorbic acid solution needed to decolorize a given volume of blue indophenol we have a measure of how much ascorbic acid is present in the solution.