Vitamin C Lab 111.doc - Bronx High School of Science Ms Boone\/Ms Stern Instructors Science Department Nutritional Science Vitamin C Lab Objective To

Vitamin C Lab 111.doc - Bronx High School of Science Ms...

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Bronx High School of Science Science Department Ms. Boone/Ms. Stern, Instructors Nutritional Science - Vitamin C Lab Objective : To test various types of juices for ascorbic acid content. Introduction : Ascorbic acid (vitamin C) is a water-soluble vitamin found in relatively high concentrations in citrus fruits such as oranges, grapefruits and lemons, as well as vegetables of the cabbage family. In its active form, it is in a reduced state, but it can be readily oxidized (lose hydrogen) to become dehydroascorbic acid. In the oxidized form it does not act as a vitamin. Oxidation 2H C 6 H 8 O 6 (Ascorbic Acid) C 6 H 6 O 6 (Dehydroascorbic Acid) 2H Reduction This property provides a method for the quantitative analysis of ascorbic acid. Using the schematic below, you can see that the amount of reduction of blue indophenol to colorless is proportional to the number of hydrogen’s contributed through the oxidation of ascorbic acid. Therefore, by counting the number of drops of ascorbic acid solution needed to decolorize a given volume of blue indophenol we have a measure of how much ascorbic acid is present in the solution.

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