WS04.docx - LIFS 2720 – Introductory Biochemical Laboratory Worksheet 4 Serum Electrophoresis Using Cellulose Acetate Name Tsoi Man Lung ID 20601836

WS04.docx - LIFS 2720 – Introductory Biochemical...

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LIFS 2720 – Introductory Biochemical Laboratory Worksheet 4 Serum Electrophoresis Using Cellulose Acetate Name: Tsoi Man Lung ID: 20601836 Group No. 15 Q1. Show the chromatogram of your membrane; identify and label each band with the corresponding separated type of serum proteins. (1.5 marks) Q2. Comment on the relative amount of serum proteins by semi-quantitative analysis using both the peak height and band colour intensity. (4 marks) From the chromatography, there are five peaks which are Gamma, Beta, Alpha 2, Alpha 1 globulins and Albumin respectively from left to right. When there are no errors during sample loading and staining the membrane, the peak height and the band colour intensity should be in direct proportional to the amount of serum proteins. That is to say, the higher the peak height or the colour intensity, the more amount of that serum protein. -ve +ve
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For the height of the peaks: Albumin>Beta=Gamma>Alpha 2>Alpha 1 For the band colour intensity: Serum protein components Colour intensity Albumin +++++
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