Properties of Enzymes.doc - Omosebi 1 Samson Omosebi BIO...

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Omosebi 1 Samson Omosebi BIO – 121 QTU Dr. Pastor Couceyro 07/14/2017 Properties of Enzymes Abstract The enzymatic activity is affected by temperature, pH, substrate concentration, and the concentration of inhibitors, inhibitors bind to the active sites of the enzymes preventing the substrates from binding on the enzymes thus reducing the rate of chemical reaction, at lower temperature, the enzymes are inactive and become denatured and permanently killed at higher temperature. Each enzyme is substrate specific and can work under a specific pH level. The experiment will determine the effect of temperature, pH, substrate concentration, and the concentration of inhibitors on the enzymatic activity of peroxide. Introduction Chemical reactions involving enzymes take place in the human body on a daily basis. The enzymes facilitate and catalyze the chemical reactions in which their absence may mean that it is not possible to have the desired reactions. Enzymes are the biological proteins that facilitate the chemical reaction process by certain levels of magnitude and orders. The enzymes achieve this property by lowering the activation energy and the amount or energy required to initiate a chemical reaction process.
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Omosebi 2 The unique amino acid sequence in the protein enzyme gives it a 3D shape coded by a particular gene unique for every enzyme. It provides enzyme their substrate specific nature, creating active sites that enable reaction sites for every chemical activity. The substrates will bind on each enzyme through an induced-fit process making the enzyme poses the ability to perform the chemical reaction. It is expected that tissue substrate concentration, temperature, pH, and inhibitors do not affect the rate of enzymatic activity. Part I: Effect of Tissue Extract Concentration on Enzymatic Activity. Materials and Methods Buffer solution at pH 5, 10mM water, 20mM guaiacol, a tissue extract kept on the ice. One 50 ml tube was obtained and filed with Buffer solution at pH 5, 10mM water, and 20mM guaiacol, a tissue extract kept on the ice and then filled up to 1/3 full. Seven additional glasses were labeled as control tube containing the tissue extract, substrate and indicator dye in tube 2, tissue extract in tube 3, substrate and indicator dye in tube 4, tissue extract in tube 5, substrate indicator dye in tube 6, and tissue extract tube 7. The tubes were recorded in a table and named table 1. Each of the seven tubes was then filed with the appropriate solution and the control tube 1 used to blank the spectrometer. Tube 2 and 3 were mixed to facilitate the enzymatic reaction and six measurements of absorbance recorded at 500 mm, a second tale. The reaction mixture was then dumped is a waste bin and then rinsed to cuvette the tube with distilled water.
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