Chem 329 (pH meter line diagram).pdf - 11/28 Lecture pH...

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Lecture 11/28 pH meter line diagram Ages , I Agcl I Ct cage , It Htcaq , outsider Htcaq , knpyy.name . I , 4- can .nu?eewm/AgClcs1/AScs , Reference electrode I glass membrane - Reference electrode 2 1) pH meter electrode examines the concentration gradient across the membrane ; not redox pairs I pH unit I 0.05924 voltage difference at 25°C . 2) pH meter measures activity , not concentration 3) sources of errors for pH meter at calibration against a pH buffer C buffer errors ) } realist pH meter accuracy I 0.01 pH b) Variations in the junction potential c) selective ion membrane not ideal ; Nat errors Chromatography C separation + analysis ) I 91192 91 t 92 basic process and terminology stationary phase C Cf ) - particle gets , polymer mobile ¢ solvent ( liquid ) , gas run the analyte through column process called " elution " analyte I ; running at different speed chromatography separation - column - Definition of chromatography : The components of a mixture are separated based on the rate at which these components are carried through a Stationary ¢ by a mobile 0 G , -192 - a , * the analyte bonds spread out - a , C diffuse ) during the elution = are process solvent = 9 , = 92 t=o t=€m t= tr t = ' r onset , tm ta y time O 9 , 92
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Principles of chromatography I separation efficiency of
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