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Emma Okawara BIOL 111-558 Photosynthesis Lab Report The purpose of this experiment was to discover the effect of different colors of light (white, dark, red, green, blue) on organisms that use photosynthesis, to learn how metabolic poisons affect photosynthesis and to interpret how these poisons affect the conversion of light and chemical energy to kinetic energy. There were three hypotheses for this experiment. The first was that blue light is the most effective at driving photosynthesis because it has the shortest wavelength so it will have a higher frequency which could help the plant absorb light faster. The second was that the motility of Chlamydomonas will decrease due to DCMU, since it is a metabolic poison so it could interfere with energy production, which is important for photosynthesis. The third was that sodium azide will decrease motility in Chlamydomonasbecause it is a highly toxic substance. Furthermore, for lab activity one, spinach leaves were cut into disks, the oxygen was removed from them with a syringe with bicarbonate solution, put into 5 beakers with sodium bicarbonate solution, and each was placed in a chamber with a different color of light. For lab activity two, first, Chlamydomonas was added to each Falcon tube, then the treatment, and the combination was added to a locomotion chamber, which then was covered with aluminum foil (half of it). After that, it was put under a light source and the spread of the Chlamydomonas was observed and recorded after ten and twenty minutes.