Review sheet - 1406 Lab Practical 1 SPRING 2019(1) (1).docx...

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BIOL 1406 Lab Practical 1 Review SheetMicroscope and Weighing1.What are the parts of a binocular compound microscope? 2.Be able to extract, stain, and focus a cheek cell in under 3 minutes.3.Be able to focus any specimen under 1 minute.4.Be able to weigh an object without including the weight of the weigh boat.5.Define field-of-view, depth-of-view, parfocal, and contrast, and be able to adjust these on a specimen, focusing up through 400XT.6.Be able to calculate total magnification.7.Explain why only part of the letter “e” was visible at 400XT.8.Be able to troubleshoot when one is having trouble focusing a specimen.pGLO Bacterial Transformation1.What is GFP? Where did it originate, and what are its applications in biotechnology?2.What is pGLO? What genes are present on it, and how are those genes turned on?3.What bacterial species did we work with? What is the general structure of this species? How does this species acquire new genes form other individuals? How do these new genes get passed on?4.What procedures were done in tubes and on each of the 4 petri dishes? Be able to do the microbiological techniques we did in lab.5.What was the purpose of the “heat shock”? How does this relate to evolution in bacteria?6.Why were the petri dishes incubated at 37C?7.What results would be expected on each of the 4 petri dishes, and why?8.Which petri dish glowed in the dark? Why? What other gene(s) was being expressed?
Virtual Biotechnology1. Explain the materials and procedures used in cheek cell DNA

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