Urine Analysis Lab.docx - Biology 20IB Urine Analysis Lab Purpose To identify kidney disorders by analyzing four urine samples Background Information

Urine Analysis Lab.docx - Biology 20IB Urine Analysis Lab...

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Biology 20IB: Urine Analysis Lab Purpose To identify kidney disorders by analyzing four urine samples Background Information The tests that will be conducted are Benedict’s Test, Biuret test and pH. Procedures 1. Drop three drops of each urine sample and water on spot plate. 2. Drop one drop of Biuret reagent in each sample of urine and water. 3. Record the colour changes of urine samples and water in data table. 4. Drop ten drops of each urine sample and water in different test tubes. 5. Drop a few drops of Benedict’s solution in each test tube with urine samples and water inside. 6. Turn on hot plate. 7. Place beaker with water inside on hot plate and wait for water to start boiling. 8. Once water is boiling, place all five test tubes inside the beaker. 9. Wait two minutes. 10. Remove test tubes from beaker and observe the colour changes of the urine samples and water. Variables Controlled Variables - amount of urine used for tests, amount of Benedict’s solution and Biuret reagent used for tests, amount of time test tubes were placed in water Manipulated Variables
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