Biochemistry Homework 3.docx - Biochemistry Homework#3(Due Wednesday Oct 2 2019 Same Homework groups 1 Explain how a lack of Vitamin C leads to scurvy

Biochemistry Homework 3.docx - Biochemistry Homework#3(Due...

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Biochemistry Homework #3 (Due Wednesday, Oct. 2, 2019) Same Homework groups 1. Explain how a lack of Vitamin C leads to scurvy. Vitamin C is important because it reduces Fe3+ to Fe2+. This means that prolyl hydroxylase is active and can convert proline to 4-hydroxyproline. Without substantial vitamin C, 4- Hydroxyproline and Hydrogen bonds are not formed. Thus, the helix is weaker and tissues with collagen are weaker as well. This leads to gum issues and skin problems due to the weakened collagen. 2. The following five proteins, which are listed with their molecular weights and isoelectric points, were separated by SDS-polyacrylamide gel electrophoresis. Give the order of their migration from the top (the point of sample application) to the bottom of the gel. 1. Transferrin 2. Serum Albumin 3. A-antitrypsin 4. Myoglobin 5. Cytochrome C 3. If the five proteins in Question 2 were separated in an isoelectric-focusing experiment, what would be their distribution between the positive (+) and negative (-) ends of the gel? Indicate the high and low pH ends. Cathode (-) _b c e a d_____________(+) Anode 1. Cytochrome C 2. Myoglobin 3. Transferrin 4. A-antitrypsin 5. Serum Albumin 4. Before high-performance liquid chromatography (HPLC) methods were devised for the separation and analysis of small peptides, electrophoresis on a paper support was frequently used. Separation was effected on the basis of the charge on a peptide at different pH values. Predict the direction of migration for the following peptides at the given pH values. Use C for migration toward the cathode, the negative pole; A for migration toward the anode, the positive pole; and O if the peptide remains stationary.
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pH pI 2.0 4.0 6.0 11.0 7.4 Lys-Gly-Ala-Gly C C C A 6.5 4 Lys-Gly-Ala-Glu C C O A 5.3 6 His-Gly-Ala-Glu C C A A 3.1 6 Glu-Gly-Ala-Glu C A A A 7.2 7 Gln-Gly-Ala-Lys C C C A 5. Many of the methods described in Chapter 3 are used to purify enzymes in their native state. Why would the use of SDS-polyacrylamide gel electrophoresis be unlikely to lead to the successful purification of an active enzyme? The use of SDS-polyacrylamide gel electrophoresis is unlikely to lead to the successful purification of an active enzyme because this method only separates proteins by size. In the
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