Group 14 EXP5.xlsx - Measurement results Practical 5 Kinetics Part 1 1:34:25 PM Absorbance 1 Wavelength 340 nm Plate 1 Reading avg time[s 2 29.99 3

Group 14 EXP5.xlsx - Measurement results Practical 5...

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Measurement results Practical 5 Kinetics Part 1 10/10/2019 1:34:25 PM Absorbance 1 Wavelength: 340 nm Plate 1 Reading avg. time [s] A01 A02 A03 A04 A05 2 29.99 1.0260 1.1021 0.9422 0.8865 0.8203 3 59.99 1.0013 1.0620 0.9023 0.8250 0.7540 4 89.99 0.9832 1.0268 0.8615 0.7631 0.7408 5 119.99 0.9648 0.9962 0.8231 0.7099 0.7296 6 149.99 0.9488 0.9701 0.7878 0.6623 0.6234 7 179.99 0.9372 0.9511 0.7548 0.6197 0.6038 8 209.99 0.9253 0.9322 0.7242 0.5792 0.5506 20.00 40.00 60.00 80.00 100.00 120.00 140.00 0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 1.2000 f(x) = − 0.001460595238095 x + 0.864185394047619 f(x) = − 0.00170630952381 x + 0.92555436547619 f(x) = − 0.0012175 x + 0.974073539285714 f(x) = − 0.000938333333333 x + 1.11837633095238 f(x) = − 0.000553214285714 x + 1.03589446785714 Graph A 66.7 μM [S] Linear (66.7 μM [S]) 133.3 μM [S] Linear (266.7 μM [S]) 400.0 μM [S] Linear (400.0 μM [S]) Time (s) A340
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Slope (A/s) 1/V0 Vmax ( 66.7 -0.0006 -1.38E-01 -7.27 0.014993 133.3 -0.0009 -2.06E-01 -4.85 0.007502 266.7 -0.0012 -2.75E-01 -3.63 0.00375 400 -0.0017 -3.90E-01 -2.57 0.0025 533.3 -0.0015 -3.44E-01 -2.91 0.001875 0 -0.006068 -2.1123 0 -0.4734176017 6.23E-03 l (cm) 0.7 avg. time [s] A01 A02 A03 A04 A05 29.99 1.0260 1.1021 0.9422 0.8865 0.8203 59.99 1.0013 1.0620 0.9023 0.8250 0.7540 89.99 0.9832 1.0268 0.8615 0.7631 0.7408 Well S µL S µmol S M S µM 1 10 0.01 6.666667E-05 66.66667 2 20 0.02 0.0001333333 133.3333 3 40 0.04 0.0002666667 266.6667 4 60 0.06 0.0004 400 5 80 0.08 0.0005333333 533.3333 S µM Slope 1/ed V0 (M/s) V0 (µM/s) 1/[S] 1/V0 66.6666666666667 -0.0007 2.29E-04 -1.61E-07 -1.61E-01 0.015 -6.23E+00 133.333333333333
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