bio199 reaction paper.docx - Bondad Saina Therese T 2015-09095 BIO199 – G6 REACTION PAPER ON BIO199 INVITED SPEAKER SEMINAR(Direct Sequencing and

bio199 reaction paper.docx - Bondad Saina Therese T...

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Bondad, Saina Therese T. 2015-09095 BIO199 – G6 REACTION PAPER ON BIO199 INVITED SPEAKER SEMINAR (Direct Sequencing and Analysis of Porcine Epidemic Diarrhea Virus Strains from Swine Farms in Luzon, Philippines in 2017 by Mr. Saubel Ezrael A. Salamat) The seminar started with a brief background on DNA sequencing and an overview of the Sanger sequencing. The speaker also provided illustrations to help us further understand the process. Next, he stated examples of next generation sequencing technologies where he mentioned Illumina, which was used in the study. He also clarified why he used Sanger and Illumina sequencing. This brief introduction became very informative for me since I have little background on Polymerase Chain Reaction(PCR) and the like since we do not conduct this in the laboratory. It helped me understand the study through a better knowledge on the process on how they obtained the results. After which, the experiment proper was discussed. A short introduction of the porcine epidemic diarrhea was done in which he characterized and stated current issues of the disease.
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