Aseptic Tube-to-Plate Lab.pdf - Aseptic Tube-to-Plate Transfers In order to complete this assignment you must purchase The Virtual Unknown Microbiology

Aseptic Tube-to-Plate Lab.pdf - Aseptic Tube-to-Plate...

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Aseptic Tube-to-Plate Transfers In order to complete this assignment you must purchase The Virtual Unknown Microbiology Internet Edition ( VUMIE ) software. For instructions on the purchase and use of this software, please return to the Module 1 Case Assignment Area. Follow the purchase instructions for either a Mac or Windows computer. You may also need to install Java and/or use a different browser if you experience difficulties when viewing any of the links for videos within the laboratory manual or assignment. Follow the instructions below. You should have answers for questions #3, #6, #7, #8, #9, #11 and #12. 1. Follow the instructions within the Laboratory Manual, Micro Digital Media TM 3/e . You will want to have this document open for reference as you complete these activities. You may want to review the introductory activities from Touring the Virtual Lab before beginning this assignment. Open the VUMIE 2012™ software. 2. Create a new unknown labeled “GPOS NEW”, using “Gram Positive Coccus” as the “subgroup”. Be sure there are NO SPACES in a label for an unknown you create! For this exercise, DO NOT check the box permitting auto-inoculation . 3. Read through the Case Study and interpret the Gram stain. 4. Obtain a DNAse Agar plate with Methyl Green from the “Media” list and provide an identifying label – dna. Appearing will be a tube of your unknown culture for inoculating the sterile plate of mint green DNAse agar. 5. Read up on DNAse agar and its use by clicking on the green “M” icon and selecting the topic from the list of media included in VirtualUnknown™ Microbiology. You will see that this medium is used for testing whether unknown bacteria can digest DNA as a nutrient for growth. For this exercise, we will streak the plate and observe the isolated colonies to determine whether DNA has been digested. This information is useful for identification. 6. Read #6 (p.13) from the laboratory manual for this exercise on tube-to-plate transfers. In this version of VirtualUnknown™ Microbiology the streak plate process is simplified to “squiggling” of the loop across the plate surface until streak lines appear. a. If you have not already done so, select DNase Agar plate from the Media dropdown list. Label your plate “dna”. b. Select the inoculating loop from the dropdown list of “Tools”.
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c. Right click on the Bunsen burner and turn it on. Place the loop in the flame until it turns red –a sign it is hot enough to have been sterilized by the flame. Note that the loop turns back to black as it “cools”.
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  • Summer '16
  • Christopher Boccio

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