PROTEIN DETECTION Dr.Sajida Parveen Shaikh
OBJECTIVES Define proteins List major body proteins in various body fluids. Proteinuria State Principle of detecting Proteins by Uristix. Check the presence of Proteins in given sample by Uristix.
Proteinuria(protein in urine) Normally, No protein is detectable in the urine. Falsely positive: *High alkaline urine sample(pH>9) *presence of High Specific gravity *after infusion with blood substitute *after intake of medication (qunine)
Risk factors of proteinuria Diabetes mellitus Hypertention Obesity Family history of Proteinuria Plus other conditions unrelated to kidney causing proteinuria are medication,trauma,toxins and infections.
Indications for quantitation of proteinuria 1)Diagnosis of Nephrotic Syndrome Traid of odema,hypoalbuminemia and proteinuria > 3g/day 2)Diagnosis of early Diabetic Nephropathy . Characterized by increase GFR,microalbuminuria and Hypertention.
Glomerular proteinuria Plasma proteins are abesent in normal urine.
- Fall '19
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