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PROTEIN DETECTION Dr.Sajida Parveen Shaikh
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OBJECTIVES Define proteins List major body proteins in various body fluids. Proteinuria State Principle of detecting Proteins by Uristix. Check the presence of Proteins in given sample by Uristix.
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Proteinuria(protein in urine) Normally, No protein is detectable in the urine. Falsely positive: *High alkaline urine sample(pH>9) *presence of High Specific gravity *after infusion with blood substitute *after intake of medication (qunine)
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Risk factors of proteinuria Diabetes mellitus Hypertention Obesity Family history of Proteinuria Plus other conditions unrelated to kidney causing proteinuria are medication,trauma,toxins and infections.
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Indications for quantitation of proteinuria 1)Diagnosis of Nephrotic Syndrome Traid of odema,hypoalbuminemia and proteinuria > 3g/day 2)Diagnosis of early Diabetic Nephropathy . Characterized by increase GFR,microalbuminuria and Hypertention.
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Glomerular proteinuria Plasma proteins are abesent in normal urine.
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