Quiz 2 objectives.docx - Hematology Quiz#2 Objectives Chapter 12 Leukocyte Development Kinetics and Functions Chapter 14 Routine and POC Testing in

Quiz 2 objectives.docx - Hematology Quiz#2 Objectives...

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Hematology Quiz #2 Objectives Chapter 12 - Leukocyte Development, Kinetics, and Functions Chapter 14 - Routine and POC Testing in Hematology Chapter 15 - Examination of Peripheral Blood Film 1. List the three granulocytes and their basic staining characteristics Ans. Eosinophil – stain with eosin as contains basic proteins (reddish orange) Basophils – contains acidic granules that stain with methylene blue (bluish) Neutrophils – react with acidic and basic stains giving them a pink or lavender color. 2. List the two mononuclear cells Ans. The mononuclear cells are – monocytes and lymphocytes. 3. Name the CD markers on the hematopoietic stem cell, the common myeloid progenitor cell, and the granulocyte-monocyte progenitor cell. Ans. Those are - Hematopoietic Stem Cells = CD34+, CD38- Common Myeloid Progenitor cells = CD34+, CD38low, CD45RA- Granulocyte Monocyte Progenitor cells = CD34+, CD38+, CD45RA+ 4. List the distinguishing characteristics of the myeloblast, promyelocyte, myelocyte, metamyelocyte, band form, and segmented neutrophil in terms of size, nucleus and cytoplasm appearance, cytoplasmic granules, and staining features. Ans. Myeloblasts: They vary in size Their nucleus is round to oval, centrally located, homogeneous with delicate chromatin, with 2-3 nucleoli. Their cytoplasm is slightly basic and their N:C ratio is 1:1 to 1:0.5. Type I has 2-4 visible nucleoli and no visible granules and Type II contains upwards of 20 granules (primary) azurophilic (burgundy). Type III have a darker chromatin with a more purple cytoplasm, contain more than 20 granules and are usually quite rare in normal bone marrow Promyelocytes: vary in size depending on the mitotic cycle. Their nucleus is round to oval and eccentric (not centrally located). There is a presence of a paranuclear halo called a “hof” in normal pros
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Their cytoplasm is evenly basophilic and filled with azurophilic primary granules. The chromatin clumping may be visible, especially around the edge and the nucleoli are easily seen. 1-3 nucleoli can be seen Neutrophil Myelocytes : The production of primary granules ceases and the cell begins to manufacture secondary or specific neutrophil granules. Neutrophil Metamyelocytes: The major morphologic change is the shape of the nucleus. Tertiary granule synthesis may occur and they are smaller than the myelocytes. The cytoplasm has very little RNA so therefore very little blue. The nucleus is indented, kidney bean shaped. The chromatin is increasingly clumped. Neutrophil Band Form: Tertiary granules continue to form and secretory granules appear. The nucleus is highly clumped and the nuclear indentations extend to greater than ½ width of a nucleus but no segments occur.
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