LR Virtual Microbe Identification .docx - Virtual Microbe...

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Virtual Microbe Identification Name Date Objectives: What kind of patient samples are used for the purpose of identifying possible pathogens? What does PCR do, how does it work, and why is it useful? How do you separate the desired DNA from all others? How does an automatic DNA sequencer work? Why is it possible to use a DNA sequence to identify bacteria? Select the link above and go through each section before finally identifying 6 samples, which are provided. Answer questions below. Questions (2 points each) Preparing the sample 1. When are molecular methods of bacterial ID preferable over traditional methods? 2. What are the basic steps of this procedure? PCR 1. What does PCR stand for? 2. What are the ingredients in mastermix? 3. What happened at the 3 temperature phases in the PCR thermocycler?
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