AsepticFA17.pdf - BIOL 2250L – Microbiology for the Health Sciences Lab#2 – Aseptic Technique and Bacterial Isolation by Streak Plate Background and

AsepticFA17.pdf - BIOL 2250L – Microbiology for the...

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BIOL 2250L – Microbiology for the Health Sciences Lab #2 – Aseptic Technique and Bacterial Isolation by Streak Plate 1 Background and Protocol Background In this laboratory experiment, you will be introduced to methods that will allow you to move, or transfer, microbial cells from one type of growth medium to another safely and efficiently. By mastering these techniques, you will be able to handle all types of microbes including pathogens with confidence. The procedures you will learn will also carry over into other parts of the medical field such as dealing with patients infected with those pathogens, without contaminating yourself, your work area, or your family and friends. 1 In this laboratory, you will learn and use a number of different terms. Inoculate is to purposely grow an organism by putting it in some media, and to contaminate is to accidentally grow an unwanted organism. Media contains all of the nutrients to grow an organism but there are no actual organisms in the media until you put them there. The common types of media are liquid broth , agar slants , agar deeps and agar plates . Agar is complex
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