Microscopy Lab Revised FA19.pdf - BIOL 2250L – Microbiology for the Heath Sciences Lab#1 – Brightfield Microscopy and Cellular Morphology Background

Microscopy Lab Revised FA19.pdf - BIOL 2250L –...

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BIOL 2250L Microbiology for the Heath Sciences Lab #1 Brightfield Microscopy and Cellular Morphology 1 Background Microbiology is study of microorganisms (microbes) and viruses. Microorganisms and viruses are too small to be seen with the naked eye. Therefore, we must use an important tool called the microscope to view microbes. There are two main types of microscopes: the light microscope and the electron microscope. We will be focusing on light microscopes in this lab exercise. The most common types of light microscopy are bright-field , dark-field , and phase- contrast . We will be using the bright-field (compound) microscope. It is called a compound microscope because it magnifies through two separate lens systems: the eyepiece and the objective . The bright-field microscope works at its best when the specimen is colored or stained. This allows for us to view the cellular morphology, cell arrangements and the results of diagnostic stains. Parts of A Microscope Stage is a platform used to hold the slide containing the specimen. On the stage, there are mechanical clips to hold the slide in place when viewing. Beneath the stage is the condenser and iris diaphragm . The condenser focuses light onto the specimen. Iris diaphragm can be used to adjust the amount of light emitted.
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