503_8-28-2019.pdf - BME‐503 Cell Molecular and Biomedical optical Imaging Lecture 2 Course Syllabus Introduction Basic Optics • Light as a particle

503_8-28-2019.pdf - BME‐503 Cell Molecular and Biomedical...

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8/28/2019 1 BME503‐Du BME‐503: Cell,MolecularandBiomedicalopticalImaging Lecture 2 Course Syllabus, Introduction, Basic Optics Light as a particle and wave Properties of light BME503‐Du BME503‐Du Bestlenssystem=oureyes! Howdoesyoureyework? Eyeisthebest opticalsystem Cones:lesssensitive, finecolors/details Rods:moresensitive([email protected]), coarsevision/night… BME503‐Du
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8/28/2019 2 BME503‐Du BME503‐Du BME503‐Du BME503‐Du Electo-Magnetic Spectrum Wave: c = λ c = speed of light =3x10 8 m/s λ = wavelength (nm) = frequency (hertz) WaveCharacteristics: E.g., blue light (λ=400 nm), what is its frequency in a vacuum? = c/ λ = 3x10 8 m/s x (10 9 nm/m )/400 nm = 7.5 x 10 14 (hz)
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8/28/2019 3 BME503‐Du BME503‐Du Wave Interference Patterns Same frequency and in phase Same frequency and out of phase Different frequency BME503‐Du Q:ifn1<n2,should i > tor i < t? BME503‐Du
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8/28/2019 4 BME503‐Du Q:ifn1>n2,should i > tor i < t? BME503‐Du TotalInternalReflection Q:ifn1>n2,then i < t BME503‐Du TotalInternalReflection(TIR) BME503‐Du TotalInternalReflection(TIR)
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