Lab 8 Addendum.docx - Nikhil Kishore Lab 8 Addendum Show your calculations for the Miller units measurement of at least one experimental strain

Lab 8 Addendum.docx - Nikhil Kishore Lab 8 Addendum Show...

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Nikhil Kishore Lab 8 Addendum Show your calculations for the Miller units measurement of at least one experimental strain. Calculations must be typed. (0.5 point) Miller Units = 1,000 x (A 420 – [1.75 x A 550 ] / (time x A 600 ) Strain A Trial 1: 1000 x (1.32 – (1.75 x .2)) / (23 x .83) = 50.81 Strain A Trial 2: 1000 x (1.93 – (1.75 x .05)) / (16 x .83) = 138.74 Recreate the data collection table (from lab manual page 78) in Excel. Make sure to include a descriptive table title. (1 point) Table 1: Data collection from strands A, B, C, and D from two trials with calculation of Miller Unites Strain Promoter A 600 Trial A 420 A 550 Start Time Stop Time Total Time (min) Miller Units A -88 to +50 0.83 1 1.32 0.2 9:16 9:29 13 89.8980538 2 1.93 0.05 9:35 9:51 16 138.74247 B -88 to +50 (no FIS binding) 0.66 1 1.12 0.46 9:30 9:50 20 23.8636364 2 0.68 0.06 9:36 9:52 16 54.4507576 C -50 to +50 0.49 1 0.19 0 9:30 9:50 20 19.3877551 2 0.07 -0.07 9:36 9:52 16 24.5535714 D No Promoter 0.69 1 0.25 0.03 9:31 9:51 20 14.3115942 2 0.24 0.22 9:37 9:53 16 -13.134058 Use Excel to create a bar graph that shows values (in Miller units) for each of the four E. coli strains. Be sure that bars on the graph represent the averaged values for duplicate experiments. Don’t forget a figure caption! (1 point)
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A B C D 0 20 40 60 80 100 120 140 Strains Average Miller Units Figure 1: The average Miller Units for each strain from two trials Questions: 1. Do the results from your experiment support your hypothesis about the role of the FIS binding site in the rR promoter? Why or why not? Explain how your results support your conclusion. (1 point)
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