Lab_3_addendum_and_instructions (1).pdf - PRINCIPLES OF BIOLOGY 216 LABORATORY 3 Nikhil Kishore Molly Shallman(Tuesday 7:45 – 10:35 Instructions As a

Lab_3_addendum_and_instructions (1).pdf - PRINCIPLES OF...

This preview shows page 1 - 4 out of 9 pages.

PRINCIPLES OF BIOLOGY 216 LABORATORY 3 Spring 2019 Nikhil Kishore Molly Shallman (Tuesday 7:45 – 10:35) April 23, 2019 Instructions As a team of four, obtain at least 250 mL of a water sample. You may provide your own water sample, or use a water sample from one of the fish tanks in the room. There will also be available water samples from a turtle tank and axolotl tank, as well as a sample from the Willamette River. Make sure that each team of four in your lab section is working with a different water sample. In pairs, you will measure the temperature and pH, and the levels of dissolved oxygen, carbon dioxide, nitrate, nitrite and ammonia. Average the measurements between the two pairs who worked on the same water sample and enter the averages into the class datasheet. The materials you need for these tests are available at different stations around the room. While you wait for specific stations to become available, answer Lab Questions 1-8. Some of the solutions we are using today are harmful. Wear gloves, safety glasses, and lab coats while conducting the following tests: A. Record the temperature of your water sample. B. Use the kit instructions at the link below to measure the dissolved oxygen in your water sample and interpret the results. C. Use the instructions below to measure the carbon dioxide in your water sample. 1. Use a graduated cylinder to measure 23 mL of your water sample. Pour the 23 mL into a beaker. 2. Add one drop of the phenolphthalein indicator solution to the beaker with the water sample. Use a stir stick to mix. 3. Add the sodium hydroxide solution drop by drop to the sample. Count each drop as it is added. Use a stir stick to mix the sample after each drop is added. Continue adding drops until a light pink color forms and persists for 30 seconds. 1 drop 4. Each drop of sodium hydroxide solution used equals 1.25 mg/l carbon dioxide. Calculate the amount, in mg/l, of carbon dioxide in your sample.
Image of page 1

Subscribe to view the full document.

PRINCIPLES OF BIOLOGY 216 LABORATORY 3 Spring 2019 D. Use the “Freshwater Master Test Kit” to measure the pH and the nitrate, nitrite and ammonia levels of your water sample. Use the instructions linked below.
Image of page 2
PRINCIPLES OF BIOLOGY 216 LABORATORY 3 Spring 2019 Lab Questions Use the information provided in the test kits and your own internet searches to answer the following questions. Don’t forget to include citations for any sources that you use! 1. Describe where your water sample was collected, and what the water environment looks like—what organisms live in the water your sample was collected from? Are there any known pollutants or chemicals that you suspect may be in your sample? (2 points) Our water sample was collected from the Willamette River. The water sample looks pretty dirty. This is because the Willamette River runs through the city of Portland and
Image of page 3

Subscribe to view the full document.

Image of page 4
  • Winter '08
  • Staff

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Ask Expert Tutors You can ask 0 bonus questions You can ask 0 questions (0 expire soon) You can ask 0 questions (will expire )
Answers in as fast as 15 minutes