DeBeurs_Step3.pdf - De Beurs Cohort November 9 2018 NAKED MOLE RAT Christina Negrete-Montero Julian Sennette Wesley Miller Katelyn Colston Ryan

DeBeurs_Step3.pdf - De Beurs Cohort November 9 2018 NAKED...

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De Beurs Cohort November 9, 2018 NAKED MOLE RAT Christina Negrete-Montero, Julian Sennette, Wesley Miller, Katelyn Colston, Ryan Hazelwood De Beurs Cohort Abstract In this study we hope to look at cellular senescent properties of Long living Heterocephalus glaber (Naked Mole Rat) (30 years), median living Fukomys Damarensis(Damaraland Mole-Rat) (15 years), and short living Georychus capensis (Cape Mole-rat) (3 years) in order to understand the relation between the cancer inhibiting properties of these rodents, free radical presence in the body/oxidative stress, and general life expectancy. Naked Mole Rats(NMR) are of particular interest due to their outlier positive correlation between the amount of reactive oxygen species (ROS) they hold and their long life expectancy whereas the other rodents in this study have a negative correlation between these two things and are close relatives to the NMR. NMRs and Damaraland Mole-Rats(DMR) eusociality is also of interest due to the differences in mitigating the effects of oxidative stress from ROS between reproductive and nonreproductive females. We will expose a culture of extracted cells from reproductive and nonreproductive females to gamma rays to induce tumor growth on a group of reproductive as well as nonreproductive NMR and DMR. We also expose the same to extracted cells from Cape Mole Rats(CMR) however they are not eusocial and as such do not require such a distinction. We will then measure the cellular senescent properties using the Cellular Senescence Flow Cytometry Assay provided by Cell Biolabs inc. to measure senescence-associated ß-galactosidase activity. Observing similar senescent responses in both reproductive and nonreproductive females would suggest a more direct correlation between senescence and ROS, while seeing an increased senescent response in reproductive females would suggest a link to cellular ability to mitigate oxidative stress rather than the ROS themselves. Objective Our objective is to identify the unique aspect of the naked mole rat DNA that results in its high resistivity to free radicals and strong resistance to cancer. By comparing the eusocial specimens (females without reproductive hormones) of the Naked Mole Rat group to the eusocial specimens of the other two species and differentiating these from their reproductive counterparts of the same species, we hope to see a difference between the levels of oxidative stress that is linked to the presence of free radicals within the body. Furthermore, by analysing how the Naked Mole Rat’s body deals with these high levels of free radicals and comparing this to the other two mole rat species, an observable difference in the specimens health is expected to be observed. Because of the positive correlation between the level of free radicals and the general health of the NMR specimens, any observable genetic differences in how they deal with high levels of free radicals is likely linked to long term health and the longevity of the lifespan of these species.
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